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GenScript corporation svnt kit genscript cpass
Svnt Kit Genscript Cpass, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genscript+cpass+kit/regf+genscript/10__1172_slash_jci181244-223-22-24
Average 90 stars, based on 1 article reviews
svnt kit genscript cpass - by Bioz Stars, 2026-09
90/100 stars

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Enzyme-linked Immunosorbent Assay:

Article Title: Validation of a SARS-CoV-2 Surrogate Neutralization Test Detecting Neutralizing Antibodies against the Major Variants of Concern
Article Snippet: .. In addition, the sVNT performed comparably to two CE-IVD-approved commercial kits, namely the Icosagen SARS-CoV-2 Neutralizing Antibody ELISA kit and the Genscript cPass kit, for the B.1 parental strain and Omicron BA.2. ..

Article Title: Validation of a SARS-CoV-2 Surrogate Neutralization Test Detecting Neutralizing Antibodies against the Major Variants of Concern.
Article Snippet: .. In addition, the sVNT performed comparably to two CE-IVDapproved commercial kits, namely the Icosagen SARS-CoV-2 Neutralizing Antibody ELISA kit and the Genscript cPass kit, for the B.1 parental strain and Omicron BA.2. ..

Control:

Article Title: Competitive ELISA for a serologic test to detect dengue serotype-specific anti-NS1 IgGs using high-affinity UB-DNA aptamers
Article Snippet: .. Detection of total SARS-CoV2 neutralizing antibodies in control human serums (a: PD0-1, b: PD02), using a GenScript cPass kit (SARS-CoV2 Surrogate Virus Neutralization Kit), before and after COVID-19 vaccination with the Pfizer-BioNTech COVID-19 mRNA-based vaccine. c. No competitive IgGs cross-reactive to dengue NS1 were detected by our competitive ELISA format. ..

Virus:

Article Title: Competitive ELISA for a serologic test to detect dengue serotype-specific anti-NS1 IgGs using high-affinity UB-DNA aptamers
Article Snippet: .. Detection of total SARS-CoV2 neutralizing antibodies in control human serums (a: PD0-1, b: PD02), using a GenScript cPass kit (SARS-CoV2 Surrogate Virus Neutralization Kit), before and after COVID-19 vaccination with the Pfizer-BioNTech COVID-19 mRNA-based vaccine. c. No competitive IgGs cross-reactive to dengue NS1 were detected by our competitive ELISA format. ..

Article Title: Suboptimal antibody response against SARS-CoV-2 Omicron variant after third dose of mRNA vaccine in kidney transplant recipients
Article Snippet: Furthermore, anti-RBD antibody OD450 values measured by ELISA for each variant and its respective neutralization percentage by surrogate virus neutralization test were moderately-to-highly correlated as well (Spearman’s ρ=0.872 and p=8.6 x10-33 for WT, Spearman’s ρ=0.800 and p=6.4 x10-24 for the Delta variant, and Spearman’s ρ=0.503 and p=7.1 x10-8 for the Omicron variant). .. Antibody neutralizing capacity: We evaluated the neutralizing function of antibodies using a surrogate virus neutralization test (GenScript cPass kit, catalog no. L00847-A), which has been shown to be highly correlated with conventional live virus neutralization testS1,8,19 and pseudovirus neutralization assays.S8,9 Patient sera (diluted 1:10) were incubated at 37C for 30 minutes with horseradish peroxidase-conjugated recombinant SARS-CoV-2 WT RBD, Delta variant RBD (HRP-RBD, GenScript catalog no. ThermoFisher catalog no. 434423) in a 1:1 ratio. .. The Delta variant RBD peptide had L452R and T478K mutations.

Neutralization:

Article Title: Competitive ELISA for a serologic test to detect dengue serotype-specific anti-NS1 IgGs using high-affinity UB-DNA aptamers
Article Snippet: .. Detection of total SARS-CoV2 neutralizing antibodies in control human serums (a: PD0-1, b: PD02), using a GenScript cPass kit (SARS-CoV2 Surrogate Virus Neutralization Kit), before and after COVID-19 vaccination with the Pfizer-BioNTech COVID-19 mRNA-based vaccine. c. No competitive IgGs cross-reactive to dengue NS1 were detected by our competitive ELISA format. ..

Article Title: Suboptimal antibody response against SARS-CoV-2 Omicron variant after third dose of mRNA vaccine in kidney transplant recipients
Article Snippet: Furthermore, anti-RBD antibody OD450 values measured by ELISA for each variant and its respective neutralization percentage by surrogate virus neutralization test were moderately-to-highly correlated as well (Spearman’s ρ=0.872 and p=8.6 x10-33 for WT, Spearman’s ρ=0.800 and p=6.4 x10-24 for the Delta variant, and Spearman’s ρ=0.503 and p=7.1 x10-8 for the Omicron variant). .. Antibody neutralizing capacity: We evaluated the neutralizing function of antibodies using a surrogate virus neutralization test (GenScript cPass kit, catalog no. L00847-A), which has been shown to be highly correlated with conventional live virus neutralization testS1,8,19 and pseudovirus neutralization assays.S8,9 Patient sera (diluted 1:10) were incubated at 37C for 30 minutes with horseradish peroxidase-conjugated recombinant SARS-CoV-2 WT RBD, Delta variant RBD (HRP-RBD, GenScript catalog no. ThermoFisher catalog no. 434423) in a 1:1 ratio. .. The Delta variant RBD peptide had L452R and T478K mutations.

Competitive ELISA:

Article Title: Competitive ELISA for a serologic test to detect dengue serotype-specific anti-NS1 IgGs using high-affinity UB-DNA aptamers
Article Snippet: .. Detection of total SARS-CoV2 neutralizing antibodies in control human serums (a: PD0-1, b: PD02), using a GenScript cPass kit (SARS-CoV2 Surrogate Virus Neutralization Kit), before and after COVID-19 vaccination with the Pfizer-BioNTech COVID-19 mRNA-based vaccine. c. No competitive IgGs cross-reactive to dengue NS1 were detected by our competitive ELISA format. ..

Incubation:

Article Title: Suboptimal antibody response against SARS-CoV-2 Omicron variant after third dose of mRNA vaccine in kidney transplant recipients
Article Snippet: Furthermore, anti-RBD antibody OD450 values measured by ELISA for each variant and its respective neutralization percentage by surrogate virus neutralization test were moderately-to-highly correlated as well (Spearman’s ρ=0.872 and p=8.6 x10-33 for WT, Spearman’s ρ=0.800 and p=6.4 x10-24 for the Delta variant, and Spearman’s ρ=0.503 and p=7.1 x10-8 for the Omicron variant). .. Antibody neutralizing capacity: We evaluated the neutralizing function of antibodies using a surrogate virus neutralization test (GenScript cPass kit, catalog no. L00847-A), which has been shown to be highly correlated with conventional live virus neutralization testS1,8,19 and pseudovirus neutralization assays.S8,9 Patient sera (diluted 1:10) were incubated at 37C for 30 minutes with horseradish peroxidase-conjugated recombinant SARS-CoV-2 WT RBD, Delta variant RBD (HRP-RBD, GenScript catalog no. ThermoFisher catalog no. 434423) in a 1:1 ratio. .. The Delta variant RBD peptide had L452R and T478K mutations.

Recombinant:

Article Title: Suboptimal antibody response against SARS-CoV-2 Omicron variant after third dose of mRNA vaccine in kidney transplant recipients
Article Snippet: Furthermore, anti-RBD antibody OD450 values measured by ELISA for each variant and its respective neutralization percentage by surrogate virus neutralization test were moderately-to-highly correlated as well (Spearman’s ρ=0.872 and p=8.6 x10-33 for WT, Spearman’s ρ=0.800 and p=6.4 x10-24 for the Delta variant, and Spearman’s ρ=0.503 and p=7.1 x10-8 for the Omicron variant). .. Antibody neutralizing capacity: We evaluated the neutralizing function of antibodies using a surrogate virus neutralization test (GenScript cPass kit, catalog no. L00847-A), which has been shown to be highly correlated with conventional live virus neutralization testS1,8,19 and pseudovirus neutralization assays.S8,9 Patient sera (diluted 1:10) were incubated at 37C for 30 minutes with horseradish peroxidase-conjugated recombinant SARS-CoV-2 WT RBD, Delta variant RBD (HRP-RBD, GenScript catalog no. ThermoFisher catalog no. 434423) in a 1:1 ratio. .. The Delta variant RBD peptide had L452R and T478K mutations.

Variant Assay:

Article Title: Suboptimal antibody response against SARS-CoV-2 Omicron variant after third dose of mRNA vaccine in kidney transplant recipients
Article Snippet: Furthermore, anti-RBD antibody OD450 values measured by ELISA for each variant and its respective neutralization percentage by surrogate virus neutralization test were moderately-to-highly correlated as well (Spearman’s ρ=0.872 and p=8.6 x10-33 for WT, Spearman’s ρ=0.800 and p=6.4 x10-24 for the Delta variant, and Spearman’s ρ=0.503 and p=7.1 x10-8 for the Omicron variant). .. Antibody neutralizing capacity: We evaluated the neutralizing function of antibodies using a surrogate virus neutralization test (GenScript cPass kit, catalog no. L00847-A), which has been shown to be highly correlated with conventional live virus neutralization testS1,8,19 and pseudovirus neutralization assays.S8,9 Patient sera (diluted 1:10) were incubated at 37C for 30 minutes with horseradish peroxidase-conjugated recombinant SARS-CoV-2 WT RBD, Delta variant RBD (HRP-RBD, GenScript catalog no. ThermoFisher catalog no. 434423) in a 1:1 ratio. .. The Delta variant RBD peptide had L452R and T478K mutations.



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Comparison of ID50 values determined by the <t>sVNT</t> <t>ELISA</t> kit and iACE2/RBD-Fc model. ID50 values of 7 vaccine serum samples and 4 convalescent plasma samples were determined by the commercial ELISA based sVNT kit and the iACE2/RBD-Fc model. A panel of raw data for determination of neutralization activity of one vaccine serum by the sVNT ELISA kit and the iACE2/RBD-Fc model was shown in (A, B) , respectively. Two sample dilution response inhibition curves generated from the data shown in Panel (A, B) were plotted and shown in (C) . ID50 values of 11 samples determined by the two assays were compared and shown in (D) . The dash line was used to indicated the serum dilution fold with an activity to inhibit 50% binding of the RBD-Fc on the ACE2 bead. p value <0.05 is represented as *.
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Comparison of ID50 values determined by the sVNT ELISA kit and iACE2/RBD-Fc model. ID50 values of 7 vaccine serum samples and 4 convalescent plasma samples were determined by the commercial ELISA based sVNT kit and the iACE2/RBD-Fc model. A panel of raw data for determination of neutralization activity of one vaccine serum by the sVNT ELISA kit and the iACE2/RBD-Fc model was shown in (A, B) , respectively. Two sample dilution response inhibition curves generated from the data shown in Panel (A, B) were plotted and shown in (C) . ID50 values of 11 samples determined by the two assays were compared and shown in (D) . The dash line was used to indicated the serum dilution fold with an activity to inhibit 50% binding of the RBD-Fc on the ACE2 bead. p value <0.05 is represented as *.

Journal: Frontiers in Immunology

Article Title: A highly sensitive bead-based flow cytometric competitive binding assay to detect SARS-CoV-2 neutralizing antibody activity

doi: 10.3389/fimmu.2022.1041860

Figure Lengend Snippet: Comparison of ID50 values determined by the sVNT ELISA kit and iACE2/RBD-Fc model. ID50 values of 7 vaccine serum samples and 4 convalescent plasma samples were determined by the commercial ELISA based sVNT kit and the iACE2/RBD-Fc model. A panel of raw data for determination of neutralization activity of one vaccine serum by the sVNT ELISA kit and the iACE2/RBD-Fc model was shown in (A, B) , respectively. Two sample dilution response inhibition curves generated from the data shown in Panel (A, B) were plotted and shown in (C) . ID50 values of 11 samples determined by the two assays were compared and shown in (D) . The dash line was used to indicated the serum dilution fold with an activity to inhibit 50% binding of the RBD-Fc on the ACE2 bead. p value <0.05 is represented as *.

Article Snippet: The ID50 (50% of maximal inhibitory dose) of samples were determined using an ELISA based sVNT kit (GenScript cPass SARS-CoV-2 neutralization Antibody detection kit, GenScript, China) according to instruction of the manufacturer with a minor modification.

Techniques: Comparison, Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Neutralization, Activity Assay, Inhibition, Generated, Binding Assay